Isolation and structural analysis of antihypertensive peptides that exist naturally in Gouda cheese

T. Saito, T. Nakamura, H. Kitazawa, Y. Kawai, T. Itoh

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    292 Citations (Scopus)

    Abstract

    Seven kinds of ripened cheeses (8-mo-aged and 24-mo-aged Gouda, Emmental, Blue, Camembert, Edam, and Havarti) were homogenized with distilled water, and water-soluble peptides were prepared by C-18 hydrophobic chromatography. The inhibitory activity to angiotensin I-converting enzyme and decrease in the systolic blood pressure in spontaneously hypertensive rats were measured before and after oral administration of each peptide sample. The strongest depressive effect in the systolic blood pressure (-24.7 mm Hg) and intensive inhibitory activity to angiotensin I-converting enzyme (75.7%) were detected in the peptides from 8-mo-aged Gouda cheese. Four peptides were isolated by HPLC with reverse-phase and gel nitration modes. Their chemical structures and origins, clarified by combination analyses of protein sequencing, amino acid composition, and mass spectrometry, were as follows: peptide A, Arg-Pro-Lys-His-Pro-Ile-Lys-His-Gln [αs1-casein (CN), B-8P; f 1-9]; peptide B, Arg-Pro-Lys-His-Pro-Ile-Lys-His-Gln-Gly-Leu-Pro-Gln (αs1CN, B-8P; f 1-13); peptide F, Tyr-Pro-Phe-Pro-Gly-Pro-Ile-Pro-Asn (β-CN, A2-5P; f 60-68); and peptide G, Met-Pro-Phe-Pro-Lys-Tyr-Pro-Val-Gln-Pro-Phe (β-CN, A2-5P; f 109-119). Peptides A and F, which were chemically synthesized, showed potent angiotensin I-converting enzyme inhibitory activity with little antihypertensive effects.

    Original languageEnglish
    Pages (from-to)1434-1440
    Number of pages7
    JournalJournal of Dairy Science
    Volume83
    Issue number7
    DOIs
    Publication statusPublished - 2000 Jul

    Keywords

    • Angiotensin-converting enzyme
    • Antihypertensivity
    • Gouda cheese
    • Peptides

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology
    • Genetics

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