Isolation and sequence determination of the chicken rhodopsin gene

Masashi Takao, Akira Yasui, Fumio Tokunaga

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

A chicken genomic library was screened with a bovine opsin cDNA probe. A clone isolated under high stringency hybridization conditions contained DNA sequences highly homologous to all of the five exons of bovine and human opsin genes. Sequence comparison of the putative open reading frame in the chicken DNA fragment of 4.3 kb with bovine opsin cDNA revealed 82% identity for the nucleotide and 87% for the deduced amino acid sequence, indicating that this DNA fragment contains the complete chicken opsin gene. The position of four introns and amino acid sequences at all putative cytoplasmic loops are exactly conserved in chicken and mammals.

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalVision Research
Volume28
Issue number4
DOIs
Publication statusPublished - 1988

Keywords

  • Genomic sequence
  • Membrane protein
  • Opsin
  • Rhodopsin
  • Sequence homology (chicken)
  • Visual pigment

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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