Isolation and sequence determination of the chicken rhodopsin gene

Masashi Takao; Akira Yasui; Fumio Tokunaga

doi:10.1016/0042-6989(88)90169-1

Isolation and sequence determination of the chicken rhodopsin gene

Masashi Takao, Akira Yasui, Fumio Tokunaga

Division of Aging Science

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

A chicken genomic library was screened with a bovine opsin cDNA probe. A clone isolated under high stringency hybridization conditions contained DNA sequences highly homologous to all of the five exons of bovine and human opsin genes. Sequence comparison of the putative open reading frame in the chicken DNA fragment of 4.3 kb with bovine opsin cDNA revealed 82% identity for the nucleotide and 87% for the deduced amino acid sequence, indicating that this DNA fragment contains the complete chicken opsin gene. The position of four introns and amino acid sequences at all putative cytoplasmic loops are exactly conserved in chicken and mammals.

Original language	English
Pages (from-to)	471-480
Number of pages	10
Journal	Vision Research
Volume	28
Issue number	4
DOIs	https://doi.org/10.1016/0042-6989(88)90169-1
Publication status	Published - 1988

Keywords

Genomic sequence
Membrane protein
Opsin
Rhodopsin
Sequence homology (chicken)
Visual pigment

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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abstract = "A chicken genomic library was screened with a bovine opsin cDNA probe. A clone isolated under high stringency hybridization conditions contained DNA sequences highly homologous to all of the five exons of bovine and human opsin genes. Sequence comparison of the putative open reading frame in the chicken DNA fragment of 4.3 kb with bovine opsin cDNA revealed 82% identity for the nucleotide and 87% for the deduced amino acid sequence, indicating that this DNA fragment contains the complete chicken opsin gene. The position of four introns and amino acid sequences at all putative cytoplasmic loops are exactly conserved in chicken and mammals.",

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T1 - Isolation and sequence determination of the chicken rhodopsin gene

AU - Takao, Masashi

AU - Yasui, Akira

AU - Tokunaga, Fumio

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N2 - A chicken genomic library was screened with a bovine opsin cDNA probe. A clone isolated under high stringency hybridization conditions contained DNA sequences highly homologous to all of the five exons of bovine and human opsin genes. Sequence comparison of the putative open reading frame in the chicken DNA fragment of 4.3 kb with bovine opsin cDNA revealed 82% identity for the nucleotide and 87% for the deduced amino acid sequence, indicating that this DNA fragment contains the complete chicken opsin gene. The position of four introns and amino acid sequences at all putative cytoplasmic loops are exactly conserved in chicken and mammals.

AB - A chicken genomic library was screened with a bovine opsin cDNA probe. A clone isolated under high stringency hybridization conditions contained DNA sequences highly homologous to all of the five exons of bovine and human opsin genes. Sequence comparison of the putative open reading frame in the chicken DNA fragment of 4.3 kb with bovine opsin cDNA revealed 82% identity for the nucleotide and 87% for the deduced amino acid sequence, indicating that this DNA fragment contains the complete chicken opsin gene. The position of four introns and amino acid sequences at all putative cytoplasmic loops are exactly conserved in chicken and mammals.

KW - Genomic sequence

KW - Membrane protein

KW - Opsin

KW - Rhodopsin

KW - Sequence homology (chicken)

KW - Visual pigment

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