Isolation and sequence analysis of the human corticotropin-releasing factor precursor gene.

S. Shibahara, Y. Morimoto, Y. Furutani, M. Notake, H. Takahashi, S. Shimizu, S. Horikawa, S. Numa

Research output: Contribution to journalArticlepeer-review

359 Citations (Scopus)


A human genomic DNA segment containing the gene for the corticotropin-releasing factor precursor has been isolated by screening a gene library with an ovine cDNA probe. The cloned DNA segment has been subjected to restriction endonuclease mapping and nucleotide sequence analysis. Comparison of the nucleotide sequence of the gene with that of the ovine cDNA indicates that an intron of 800 bp is inserted in the segment encoding the 5'-untranslated region of the mRNA. The segment corresponding to the protein-coding and the 3'-untranslated region of the mRNA is uninterrupted. The mRNA and amino acid sequences of the human corticotropin-releasing factor precursor have been deduced from the corresponding gene sequence. The deduced amino acid sequence of human corticotropin-releasing factor exhibits seven amino acid substitutions in comparison with the ovine counterpart.

Original languageEnglish
Pages (from-to)775-779
Number of pages5
JournalThe EMBO journal
Issue number5
Publication statusPublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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