Isolation and primary structure of endorphin from salmon pituitary glands

Hiroshi Kawauchi, Makoto Tubokawa, Koji Muramoto

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)


    Endorphin has been isolated from an acid acetone extract of the pituitary of the salmon Oncorhynchus keta by ion exchange chromatography and gel filtration. Sequence analysis revealed it to be a nonacosapeptide with following primary structure: Ac-Tyr-Gly-Gly-Phe-Met-Lys-Pro-Tyr-Thr-Lys-Gln-Ser-His-Lys-Pro-Leu-Ile-Thr-Leu-Leu-Lys-His- Ile-Thr-Leu-Lys-Asn-Glu-Gln-OH. It appears that the amino terminal segment which is necessary for analgesic activity is conserved through the evolution of vertebrate except for the blocking of the amino terminal of salmon endorphin.

    Original languageEnglish
    Pages (from-to)1249-1254
    Number of pages6
    JournalBiochemical and biophysical research communications
    Issue number4
    Publication statusPublished - 1979 Jun 27

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology


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