Isolation and primary structure of endorphin from salmon pituitary glands

Hiroshi Kawauchi; Makoto Tubokawa; Koji Muramoto

doi:10.1016/0006-291X(79)91114-8

Isolation and primary structure of endorphin from salmon pituitary glands

Hiroshi Kawauchi, Makoto Tubokawa, Koji Muramoto

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Endorphin has been isolated from an acid acetone extract of the pituitary of the salmon Oncorhynchus keta by ion exchange chromatography and gel filtration. Sequence analysis revealed it to be a nonacosapeptide with following primary structure: Ac-Tyr-Gly-Gly-Phe-Met-Lys-Pro-Tyr-Thr-Lys-Gln-Ser-His-Lys-Pro-Leu-Ile-Thr-Leu-Leu-Lys-His- Ile-Thr-Leu-Lys-Asn-Glu-Gln-OH. It appears that the amino terminal segment which is necessary for analgesic activity is conserved through the evolution of vertebrate except for the blocking of the amino terminal of salmon endorphin.

Original language	English
Pages (from-to)	1249-1254
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	88
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(79)91114-8
Publication status	Published - 1979 Jun 27

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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T1 - Isolation and primary structure of endorphin from salmon pituitary glands

AU - Kawauchi, Hiroshi

AU - Tubokawa, Makoto

AU - Muramoto, Koji

PY - 1979/6/27

Y1 - 1979/6/27

N2 - Endorphin has been isolated from an acid acetone extract of the pituitary of the salmon Oncorhynchus keta by ion exchange chromatography and gel filtration. Sequence analysis revealed it to be a nonacosapeptide with following primary structure: Ac-Tyr-Gly-Gly-Phe-Met-Lys-Pro-Tyr-Thr-Lys-Gln-Ser-His-Lys-Pro-Leu-Ile-Thr-Leu-Leu-Lys-His- Ile-Thr-Leu-Lys-Asn-Glu-Gln-OH. It appears that the amino terminal segment which is necessary for analgesic activity is conserved through the evolution of vertebrate except for the blocking of the amino terminal of salmon endorphin.

AB - Endorphin has been isolated from an acid acetone extract of the pituitary of the salmon Oncorhynchus keta by ion exchange chromatography and gel filtration. Sequence analysis revealed it to be a nonacosapeptide with following primary structure: Ac-Tyr-Gly-Gly-Phe-Met-Lys-Pro-Tyr-Thr-Lys-Gln-Ser-His-Lys-Pro-Leu-Ile-Thr-Leu-Leu-Lys-His- Ile-Thr-Leu-Lys-Asn-Glu-Gln-OH. It appears that the amino terminal segment which is necessary for analgesic activity is conserved through the evolution of vertebrate except for the blocking of the amino terminal of salmon endorphin.

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Isolation and primary structure of endorphin from salmon pituitary glands

Abstract

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