Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic Skunk cabbage (Symplocarpus renifolius)

Yasuko Ito-Inaba; Hiromi Masuko; Masao Watanabe; Takehito Inaba

doi:10.1271/bbb.120434

Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic Skunk cabbage (Symplocarpus renifolius)

Yasuko Ito-Inaba, Hiromi Masuko, Masao Watanabe, Takehito Inaba

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.

Original language	English
Pages (from-to)	1990-1992
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	76
Issue number	10
DOIs	https://doi.org/10.1271/bbb.120434
Publication status	Published - 2012

Keywords

Cysteine protease
Floral development
Symplocarpus renifolius
Thermogenesis

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.120434

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title = "Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic Skunk cabbage (Symplocarpus renifolius)",

abstract = "Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.",

keywords = "Cysteine protease, Floral development, Symplocarpus renifolius, Thermogenesis",

author = "Yasuko Ito-Inaba and Hiromi Masuko and Masao Watanabe and Takehito Inaba",

note = "Funding Information: We thank Ms. Fumi Adachi (University of Miyazaki) for technical support. This work was supported by Grants-in-Aid for Challenging Exploratory Research (no. 23658094), the Program to Disseminate Tenure Tracking System from the Japanese Ministry of Education, Culture, Sports, Science and Technology, and by a grant for Scientific Research on Priority Areas from the University of Miyazaki.",

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language = "English",

volume = "76",

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T1 - Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic Skunk cabbage (Symplocarpus renifolius)

AU - Ito-Inaba, Yasuko

AU - Masuko, Hiromi

AU - Watanabe, Masao

AU - Inaba, Takehito

N1 - Funding Information: We thank Ms. Fumi Adachi (University of Miyazaki) for technical support. This work was supported by Grants-in-Aid for Challenging Exploratory Research (no. 23658094), the Program to Disseminate Tenure Tracking System from the Japanese Ministry of Education, Culture, Sports, Science and Technology, and by a grant for Scientific Research on Priority Areas from the University of Miyazaki.

PY - 2012

Y1 - 2012

N2 - Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.

AB - Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.

KW - Cysteine protease

KW - Floral development

KW - Symplocarpus renifolius

KW - Thermogenesis

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VL - 76

SP - 1990

EP - 1992

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 10

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Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic Skunk cabbage (Symplocarpus renifolius)

Abstract

Keywords

ASJC Scopus subject areas

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