Abstract
We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.
Original language | English |
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Pages (from-to) | 319-323 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 269 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1990 Sept 3 |
Keywords
- Alternative splicing
- Functional expression
- Peptide α-amidating enzyme
- Secretory form
- Transmembrane domain
- mRNA
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology