Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

Ichiro Kato; Hideto Yonekura; Hiroshi Yamamoto; Hiroshi Okamoto

doi:10.1016/0014-5793(90)81184-P

Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

Ichiro Kato, Hideto Yonekura, Hiroshi Yamamoto, Hiroshi Okamoto

Surgery

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

Original language	English
Pages (from-to)	319-323
Number of pages	5
Journal	FEBS Letters
Volume	269
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(90)81184-P
Publication status	Published - 1990 Sept 3

Keywords

Alternative splicing
Functional expression
Peptide α-amidating enzyme
Secretory form
Transmembrane domain
mRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(90)81184-P

Cite this

@article{4e063a2a0cfb401db280774f5cfcd889,

title = "Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain",

abstract = "We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.",

keywords = "Alternative splicing, Functional expression, Peptide α-amidating enzyme, Secretory form, Transmembrane domain, mRNA",

author = "Ichiro Kato and Hideto Yonekura and Hiroshi Yamamoto and Hiroshi Okamoto",

year = "1990",

month = sep,

day = "3",

doi = "10.1016/0014-5793(90)81184-P",

language = "English",

volume = "269",

pages = "319--323",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

AU - Kato, Ichiro

AU - Yonekura, Hideto

AU - Yamamoto, Hiroshi

AU - Okamoto, Hiroshi

PY - 1990/9/3

Y1 - 1990/9/3

N2 - We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

AB - We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

KW - Alternative splicing

KW - Functional expression

KW - Peptide α-amidating enzyme

KW - Secretory form

KW - Transmembrane domain

KW - mRNA

UR - http://www.scopus.com/inward/record.url?scp=0025075252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025075252&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(90)81184-P

DO - 10.1016/0014-5793(90)81184-P

M3 - Article

C2 - 2401356

AN - SCOPUS:0025075252

SN - 0014-5793

VL - 269

SP - 319

EP - 323

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this