Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

Ichiro Kato, Hideto Yonekura, Hiroshi Yamamoto, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

Original languageEnglish
Pages (from-to)319-323
Number of pages5
JournalFEBS Letters
Volume269
Issue number2
DOIs
Publication statusPublished - 1990 Sep 3

Keywords

  • Alternative splicing
  • Functional expression
  • Peptide α-amidating enzyme
  • Secretory form
  • Transmembrane domain
  • mRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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