Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

Ichiro Kato; Hideto Yonekura; Hiroshi Yamamoto; Hiroshi Okamoto

doi:10.1016/0014-5793(90)81184-P

Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

Ichiro Kato, Hideto Yonekura, Hiroshi Yamamoto, Hiroshi Okamoto

Surgery

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

Original language	English
Pages (from-to)	319-323
Number of pages	5
Journal	FEBS Letters
Volume	269
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(90)81184-P
Publication status	Published - 1990 Sep 3

Keywords

Alternative splicing
Functional expression
Peptide α-amidating enzyme
Secretory form
Transmembrane domain
mRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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T1 - Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain

AU - Kato, Ichiro

AU - Yonekura, Hideto

AU - Yamamoto, Hiroshi

AU - Okamoto, Hiroshi

PY - 1990/9/3

Y1 - 1990/9/3

N2 - We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

AB - We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

KW - Alternative splicing

KW - Functional expression

KW - Peptide α-amidating enzyme

KW - Secretory form

KW - Transmembrane domain

KW - mRNA

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