TY - JOUR
T1 - Isolation and functional expression of pituitary peptidylglycine α-amidating enzyme mRNA. A variant lacking the transmembrane domain
AU - Kato, Ichiro
AU - Yonekura, Hideto
AU - Yamamoto, Hiroshi
AU - Okamoto, Hiroshi
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1990/9/3
Y1 - 1990/9/3
N2 - We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.
AB - We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.
KW - Alternative splicing
KW - Functional expression
KW - Peptide α-amidating enzyme
KW - Secretory form
KW - Transmembrane domain
KW - mRNA
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U2 - 10.1016/0014-5793(90)81184-P
DO - 10.1016/0014-5793(90)81184-P
M3 - Article
C2 - 2401356
AN - SCOPUS:0025075252
VL - 269
SP - 319
EP - 323
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -