Isolation and expression of a cDNA encoding a male-specific rat sulfotransferase that catalyzes activation of N-hydroxy-2-acetylaminofluorene

K. Nagata, S. Ozawa, M. Miyata, M. Shimada, D. W. Gong, Y. Yamazoe, R. Kato

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    110 Citations (Scopus)

    Abstract

    A cDNA (ST1C1 cDNA) encoding a N-hydroxyarylamine sulfotransferase (HAST- I) was isolated from a liver cDNA library of a male adult rat and was expressed in COS-1 cells. ST1C1 cDNA (1363 base pairs) encoded a protein of 304 amino acids with a molecular mass of 35,768 daltons, which shared 50.7 and 46.1% sequence identity with rat aryl (ST1A1 (PST-1)) and estrogen (rOST) sulfotransferases, respectively. N-terminal amino acid sequences of three digested polypeptide fragments of HAST-I were completely identical with two portions of the ST1C1 amino acid sequence. The profile of age- and sex- related expression of ST1C1 mRNA was quite consistent with changes in the sulfating activity of N-hydroxyarylamine and HAST contents in rat livers. ST1C1 expressed in COS-1 cells catalyzed a sulfation of N-hydroxy-2- acetylaminofluorene (N-OH-AAF) at a rate of 4.98 nmol/mg of protein/min and mediated PAPS (3'-phosphoadenosine-5'-phosphosulfate)-dependent DNA binding of N-OH-AAF. Although ASTIV was believed to be responsible for the activation of N-OH-AAF, ST1A1 encoding an arylsulfotransferase ASTIV, showed only a marginal activity in a sulfation and covalent binding of N-OH-AAF. These data clearly indicate that ST1C1 cDNA codes a new form of a male-dominant sulfotransferase (HAST) responsible for the bioactivation of N- hydroxyarylamines in rat livers.

    Original languageEnglish
    Pages (from-to)24720-24725
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume268
    Issue number33
    Publication statusPublished - 1993 Jan 1

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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