Isolation and comparison of different ecdysone-responsive cuticle protein genes in wing discs of Bombyx mori

Takanori Noji, Manabu Ote, Masahisa Takeda, Kazuei Mita, Toru Shimada, Hideki Kawasaki

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Microarray analysis was used to isolate an ecdysone up-regulated cuticle protein gene from wing discs of Bombyx mori. Transcripts of isolated cDNAs were identified by Northern blot analysis. Expression of the BMWCP10 gene was observed during the W0-W3 stages with the strongest signal being at the W2 stage. In contrast, expression of the BMWCP2 gene was observed at the W3and P0 stages. Expression of BMWCP10 was identified after exposure to 20E in vitro, while that of BMWCP2 was identified after exposure to 20E followed by its removal. Induction of BMWCP10 by 20E was observed in 30 min and was not inhibited by cycloheximide. Expression of BMWCP2 was observed in wing discs cultured for more than 18 h in a hormone-free medium after 20E removal. At least 4 h exposure to 20E was required before removal for induction of BMWCP2. Induction of BMWCP2 required protein synthesis. Thus, different ecdysone-responsive cuticle protein genes in wing discs of Bombyx mori were isolated.

Original languageEnglish
Pages (from-to)671-679
Number of pages9
JournalInsect Biochemistry and Molecular Biology
Volume33
Issue number7
DOIs
Publication statusPublished - 2003 Jul 1

Keywords

  • Bombyx mori
  • Cuticle protein
  • Ecdysone
  • Metamorphosis
  • Microarray analysis
  • Wing disc

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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