Isolation and co-cultivation of human macrophages and neutrophils with Plasmodium falciparum-parasitized erythrocytes: An optimized system to study the phagocytic activity to malarial parasites

Mohamad Alaa Terkawi, Ryo Takano, Kentaro Kato

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Macrophages and neutrophils are our front line of defense against invading pathogens. They are professional phagocytic cells that play a key role in the clearance of Plasmodium falciparum-parasitized erythrocytes from the host circulation. A stable in vitro culture system for these cells and parasitized erythrocytes would provide the means to study their immunological responses to infection, potentially revealing important clues for new therapeutic interventions for malaria. Here, we present an optimized protocol for cultivating human peripheral blood monocyte-derived macrophages and neutrophils with Plasmodium falciparum-parasitized erythrocytes.

Original languageEnglish
Pages (from-to)545-548
Number of pages4
JournalParasitology International
Volume65
Issue number5
DOIs
Publication statusPublished - 2016 Oct 1

Keywords

  • Macrophage
  • Neutrophil
  • Phagocytosis
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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