Isolation and characterization of vasohibin-2 as a homologue of VEGF-inducible endothelium-derived angiogenesis inhibitor vasohibin

Takumi Shibuya, Kazuhide Watanabe, Hiroshi Yamashita, Kazue Shimizu, Hiroki Miyashita, Mayumi Abe, Takuya Moriya, Hideki Ohta, Hikaru Sonoda, Tooru Shimosegawa, Koichi Tabayashi, Yasufumi Sato

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66 Citations (Scopus)

Abstract

Objective - We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile. Methods and Results - By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice. Conclusion - These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors.

Original languageEnglish
Pages (from-to)1051-1057
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume26
Issue number5
DOIs
Publication statusPublished - 2006 May

Keywords

  • Angiogenesis inhibitor
  • Endothelial cells
  • Vascular development
  • Vasohibin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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    Shibuya, T., Watanabe, K., Yamashita, H., Shimizu, K., Miyashita, H., Abe, M., Moriya, T., Ohta, H., Sonoda, H., Shimosegawa, T., Tabayashi, K., & Sato, Y. (2006). Isolation and characterization of vasohibin-2 as a homologue of VEGF-inducible endothelium-derived angiogenesis inhibitor vasohibin. Arteriosclerosis, thrombosis, and vascular biology, 26(5), 1051-1057. https://doi.org/10.1161/01.ATV.0000216747.66660.26