Isolation and characterization of twenty microsatellite loci in Japanese sea cucumber (Stichopus japonicus)

Manami Kanno, Qi Li, Akihiro Kijima

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36 Citations (Scopus)

Abstract

Twenty microsatellite markers were first developed from the Japanese sea cucumber Stichopus japonicus using an enrichment protocol. Of the 20 microsatellite loci, 19 loci were polymorphic in the population examined. At these polymorphic loci, the number of alleles per locus varied from 2 to 15, and the observed heterozygosities ranged from 0.03 to 0.97, which is considerably higher than those previously found for allozymes. The high variability of the microsatellite markers identified in this study will make them excellent tools for genetic analyses of S. japonicus.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalMarine Biotechnology
Volume7
Issue number3
DOIs
Publication statusPublished - 2005 Jun 1

Keywords

  • Genetic variability
  • Microsatellite loci
  • Sea cucumber
  • Stichopus japonicus

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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