TY - JOUR
T1 - Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification
AU - Yun-Guo Liu, Liu
AU - Han, Qing Dian
AU - Li, Yao Yao
AU - Liu, Ling Xiao
AU - Li, Yu You
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Abstract: Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (H O ) and expected (H E ) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species.
AB - Abstract: Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (H O ) and expected (H E ) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species.
KW - Aspergillus niger
KW - RAPD
KW - microsatellite marker
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U2 - 10.3103/S0891416818040043
DO - 10.3103/S0891416818040043
M3 - Article
AN - SCOPUS:85062302510
VL - 33
SP - 254
EP - 260
JO - Molecular Genetics, Microbiology and Virology
JF - Molecular Genetics, Microbiology and Virology
SN - 0891-4168
IS - 4
ER -