Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification

Liu Yun-Guo Liu, Qing Dian Han, Yao Yao Li, Ling Xiao Liu, Yu You Li

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (H O ) and expected (H E ) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalMolecular Genetics, Microbiology and Virology
Volume33
Issue number4
DOIs
Publication statusPublished - 2018 Oct 1

Keywords

  • Aspergillus niger
  • RAPD
  • microsatellite marker

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics
  • Virology
  • Infectious Diseases

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