Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification

Liu Yun-Guo Liu; Qing Dian Han; Yao Yao Li; Ling Xiao Liu; Yu You Li

doi:10.3103/S0891416818040043

Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification

Liu Yun-Guo Liu, Qing Dian Han, Yao Yao Li, Ling Xiao Liu, Yu You Li

ENG - Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (H _O ) and expected (H _E ) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species.

Original language	English
Pages (from-to)	254-260
Number of pages	7
Journal	Molecular Genetics, Microbiology and Virology
Volume	33
Issue number	4
DOIs	https://doi.org/10.3103/S0891416818040043
Publication status	Published - 2018 Oct 1

Keywords

Aspergillus niger
RAPD
microsatellite marker

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3103/S0891416818040043

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Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification. / Yun-Guo Liu, Liu; Han, Qing Dian; Li, Yao Yao et al.

In: Molecular Genetics, Microbiology and Virology, Vol. 33, No. 4, 01.10.2018, p. 254-260.

Research output: Contribution to journal › Article › peer-review

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title = "Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification",

abstract = " Abstract: Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (H O ) and expected (H E ) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species. ",

keywords = "Aspergillus niger, RAPD, microsatellite marker",

author = "{Yun-Guo Liu}, Liu and Han, {Qing Dian} and Li, {Yao Yao} and Liu, {Ling Xiao} and Li, {Yu You}",

note = "Funding Information: This work was supported by grants from the High-level talent introduction project of Xinjiang Uygur Autonomous Region (201412) and the project of domestic visiting scholars for young College Backbone Teachers in Shandong province (SD2014).",

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AU - Li, Yao Yao

AU - Liu, Ling Xiao

AU - Li, Yu You

N1 - Funding Information: This work was supported by grants from the High-level talent introduction project of Xinjiang Uygur Autonomous Region (201412) and the project of domestic visiting scholars for young College Backbone Teachers in Shandong province (SD2014).

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N2 - Abstract: Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (H O ) and expected (H E ) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species.

AB - Abstract: Thirty polymorphic simple sequence repeat (SSR) markers have been developed and characterized from RAPD product in Aspergillus niger in order to protect its natural resources. Polymorphisms of these RAPD-SSR markers were evaluated in a natural population of 25 strains collected from Shandong in China. The number of alleles (Na) per locus varied from 2 to 9. Observed (H O ) and expected (H E ) heterozygosities ranged from 0.56 to 0.96, and from 0.52 to 0.88, respectively. All the RAPD-SSR loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. They have showed sufficient level of polymorphisms to estimate the genetic diversity, population structure and species conservation in the A. niger. Five additional fungus species, Penicillium chrysogenum, Penicillium citrinum, Aspergillus flavus, Aspergillus ochratoxin and Aspergillus sulphureus were assessed for cross-species amplification. Three of the five species showed at least three polymorphic loci. In addition, eight loci were found to be polymorphic in at least one species.

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Isolation and Characterization of Thirty Polymorphic Microsatellite Markers from RAPD Product in Aspergillus niger and a Test of Cross-Species Amplification

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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