Isolation and characterization of the human gene homologous to the Drosophila headcase (hdc) gene in chromosome bands 6q23-q24, a region of common deletion in human pancreatic cancer

Naohiko Makino, Takashi Yamato, Hiroko Inoue, Toru Furukawa, Tadayoshi Abe, Tadaaki Yokoyama, Toshimasa Yatsuoka, Shinichi Fukushige, Seiichi Orikasa, Tsuneo Takahashi, Akira Horii

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Deletions of the long arm of chromosome 6 (6q) are one of the most common chromosomal abnormalities in multiple human malignancies. Previously, we have identified three commonly deleted regions on 6q (6q21, 6q23-q24, and 6q26) in pancreatic cancer by loss of heterozygosity studies, suggesting the presence of one or more tumor suppressor genes on this chromosome arm. Using a combination of database search and cDNA library screening, we successfully isolated a transcript from 6q24. This mRNA encodes a protein consisting of 543 amino acids with homology to the Drosophila headcase (hdc) gene and, thus, is designated as hHDC. Northern analysis identified a ubiquitously expressed 5.6-kb transcript. Seventeen (81%) of 21 pancreatic cancer cell lines and four (80%) of five renal cell carcinoma cell lines showed low level expression, suggesting that the hHDC gene may play an important role in some human cancers.

Original languageEnglish
Pages (from-to)547-553
Number of pages7
JournalMitochondrial DNA
Volume11
Issue number6
DOIs
Publication statusPublished - 2001

Keywords

  • chromosome arm 6q
  • human pancreatic cancer
  • the headcase gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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