Isolation and characterization of microsatellites in the Asian walking catfish Clarias macrocephalus

S. Sukmanomon, Supawadee Poompuang, Masamichi Nakajima

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microsatellite loci were characterized in the walking catfish, Clarias macrocephalus, random clones from a small genomic library using a (GT) 15 probe. Primers for DNA amplifications using polymerase chain reaction (PCR) were designed and synthesized for 23 loci. Twelve loci were polymorphic, with the number of alleles ranging from two to 13 alleles per locus. Developed microsatellite primers should prove useful for population studies and genetic mapping of the walking catfish.

Original languageEnglish
Pages (from-to)350-351
Number of pages2
JournalMolecular Ecology Notes
Volume3
Issue number3
DOIs
Publication statusPublished - 2003 Sep 1

Keywords

  • Clarias macrocephalus
  • Genetic mapping
  • Heterozygosity
  • Microsatellites

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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