Isolation and characterization of microsatellites in the Asian walking catfish Clarias macrocephalus

S. Sukmanomon; Supawadee Poompuang; Masamichi Nakajima

doi:10.1046/j.1471-8286.2003.00446.x

Isolation and characterization of microsatellites in the Asian walking catfish Clarias macrocephalus

S. Sukmanomon, Supawadee Poompuang, Masamichi Nakajima

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Microsatellite loci were characterized in the walking catfish, Clarias macrocephalus, random clones from a small genomic library using a (GT) ₁₅ probe. Primers for DNA amplifications using polymerase chain reaction (PCR) were designed and synthesized for 23 loci. Twelve loci were polymorphic, with the number of alleles ranging from two to 13 alleles per locus. Developed microsatellite primers should prove useful for population studies and genetic mapping of the walking catfish.

Original language	English
Pages (from-to)	350-351
Number of pages	2
Journal	Molecular Ecology Notes
Volume	3
Issue number	3
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00446.x
Publication status	Published - 2003 Sep 1

Keywords

Clarias macrocephalus
Genetic mapping
Heterozygosity
Microsatellites

ASJC Scopus subject areas

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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AU - Sukmanomon, S.

AU - Poompuang, Supawadee

AU - Nakajima, Masamichi

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Microsatellite loci were characterized in the walking catfish, Clarias macrocephalus, random clones from a small genomic library using a (GT) 15 probe. Primers for DNA amplifications using polymerase chain reaction (PCR) were designed and synthesized for 23 loci. Twelve loci were polymorphic, with the number of alleles ranging from two to 13 alleles per locus. Developed microsatellite primers should prove useful for population studies and genetic mapping of the walking catfish.

AB - Microsatellite loci were characterized in the walking catfish, Clarias macrocephalus, random clones from a small genomic library using a (GT) 15 probe. Primers for DNA amplifications using polymerase chain reaction (PCR) were designed and synthesized for 23 loci. Twelve loci were polymorphic, with the number of alleles ranging from two to 13 alleles per locus. Developed microsatellite primers should prove useful for population studies and genetic mapping of the walking catfish.

KW - Clarias macrocephalus

KW - Genetic mapping

KW - Heterozygosity

KW - Microsatellites

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Isolation and characterization of microsatellites in the Asian walking catfish Clarias macrocephalus

Abstract

Keywords

ASJC Scopus subject areas

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