Isolation and characterization of microsatellite markers from Tiarella polyphylla (saxifragaceae)

Keum Seon Jeong; Masayuki Maki; Jae Hong Pak

doi:10.3732/ajb.1100553

Isolation and characterization of microsatellite markers from Tiarella polyphylla (saxifragaceae)

Keum Seon Jeong, Masayuki Maki, Jae Hong Pak

Botanical Gardens

Research output: Contribution to journal › Article › peer-review

Abstract

Premise of the study: Microsatellite markers were developed for Tiarella polyphylla in Korea and Japan to study the genetic diversity and genetic structure of the species. • Methods and Results: We successfully isolated a total of nine polymorphic simple sequence repeat loci. These nine loci contained from two to eight alleles per locus. The observed and expected heterozygosities ranged from 0.000 to 0.250 and 0.000 to 0.974, respectively. • Conclusions: The markers developed in this study may be used to estimate genetic diversity, genetic structure, and historical and recent gene flow in T. polyphylla, which requires conservation and management.

Original language	English
Pages (from-to)	255-257
Number of pages	3
Journal	American Journal of Botany
Volume	99
Issue number	6
DOIs	https://doi.org/10.3732/ajb.1100553
Publication status	Published - 2012 Jun 1

Keywords

Microsatellites
Population genetics
Saxifragaceae
Tiarella polyphylla

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics
Plant Science

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10.3732/ajb.1100553

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abstract = "Premise of the study: Microsatellite markers were developed for Tiarella polyphylla in Korea and Japan to study the genetic diversity and genetic structure of the species. • Methods and Results: We successfully isolated a total of nine polymorphic simple sequence repeat loci. These nine loci contained from two to eight alleles per locus. The observed and expected heterozygosities ranged from 0.000 to 0.250 and 0.000 to 0.974, respectively. • Conclusions: The markers developed in this study may be used to estimate genetic diversity, genetic structure, and historical and recent gene flow in T. polyphylla, which requires conservation and management.",

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AU - Jeong, Keum Seon

AU - Maki, Masayuki

AU - Pak, Jae Hong

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Y1 - 2012/6/1

N2 - Premise of the study: Microsatellite markers were developed for Tiarella polyphylla in Korea and Japan to study the genetic diversity and genetic structure of the species. • Methods and Results: We successfully isolated a total of nine polymorphic simple sequence repeat loci. These nine loci contained from two to eight alleles per locus. The observed and expected heterozygosities ranged from 0.000 to 0.250 and 0.000 to 0.974, respectively. • Conclusions: The markers developed in this study may be used to estimate genetic diversity, genetic structure, and historical and recent gene flow in T. polyphylla, which requires conservation and management.

AB - Premise of the study: Microsatellite markers were developed for Tiarella polyphylla in Korea and Japan to study the genetic diversity and genetic structure of the species. • Methods and Results: We successfully isolated a total of nine polymorphic simple sequence repeat loci. These nine loci contained from two to eight alleles per locus. The observed and expected heterozygosities ranged from 0.000 to 0.250 and 0.000 to 0.974, respectively. • Conclusions: The markers developed in this study may be used to estimate genetic diversity, genetic structure, and historical and recent gene flow in T. polyphylla, which requires conservation and management.

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KW - Population genetics

KW - Saxifragaceae

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Isolation and characterization of microsatellite markers from Tiarella polyphylla (saxifragaceae)

Abstract

Keywords

ASJC Scopus subject areas

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