Isolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai

Qi Li, Choulji Park, Akihiro Kijima

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117 Citations (Scopus)

Abstract

Four microsatellite loci, designated Hdh1321, Hdh78, Hdh1761, and Hdh1457, were isolated from the Pacific abalone, Haliotis discus hannai, using an enrichment method based on magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. Primers designed to amplify via polymerase chain reaction the microsatellite loci were used to screen 30 individuals from a natural Pacific abalone population in Onagawa Bay, Miyagi Prefecture, Japan. The four microsatellite loci were all polymorphic, with an average of 14.3 alleles per locus (range 7-20). The mean observed and expected heterozygosities were 0.48 (range 0.30-0.97) and 0.79 (range 0.60-0.92), respectively. Significant deviations from Hardy-Weinberg expectations were observed at three loci as a result of homozygote excess. The expected heterozygosity values were considerably higher than those previously found for allozymes (range 0.101-0.125), suggesting that these microsatellite loci should provide useful markers for studies of trait mapping, kinship, and population genetics.

Original languageEnglish
Pages (from-to)811-815
Number of pages5
JournalJournal of Shellfish Research
Volume21
Issue number2
Publication statusPublished - 2002 Dec 1

Keywords

  • Genetic variability
  • Haliotis discus hannai
  • Microsatellite loci
  • Pacific abalone

ASJC Scopus subject areas

  • Aquatic Science

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