Isolation and characterization of microsatellite DNA loci from the golden cuttlefish, Sepia esculenta Hoyle (Cephalopoda)

Xiaodong Zheng, Minoru Ikeda, Anna Barinova, Nobuhiko Taniguchi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The first microsatellite markers were isolated from the golden cuttlefish, Sepia esculenta Hoyle. Eleven primer sets were designed to amplify the marker sequences via polymerase chain reaction. The 45-50 individuals from one wild population in the coastal waters of Ehime Prefecture, Japan were used to screen polymorphism in the 11 microsatellite loci. All the microsatellite loci were polymorphic, with the range of alleles from seven to 27 per locus. The observed and expected heterozygosities ranged from 0.380 to 0.980 and from 0.654 to 0.940, respectively. These marker loci except for one locus showing significant deviation from Hardy-Weinberg equilibrium will be useful for the assessment of genetic variation and population structure of this species.

Original languageEnglish
Pages (from-to)40-42
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Keywords

  • Cephalopoda
  • Cuttlefish
  • Genetic variation
  • Microsatellite DNA
  • Sepia esculenta

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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