Isolation and characterization of microsatellite DNA loci for endangered fish, Japanese huchen (Hucho perryi)

Makoto Hatakeyama, Tomohisa Watanabe, Minoru Ikeda, Masamichi Nakajima, Hiroshi Kawamula, Nobuhiko Taniguchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Japanese huchen, Hucho perryi, is an endangered fish in Japan. In order to promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. For this purpose, we designed 15 primers containing dinucleotide microsatellite regions and examined their allelic variation using a total of 23 wild individuals collected from two different localities. Eight loci showed moderate allelic variation ranging from two to four alleles, with expected heterozygosities from 0.043 to 0.548. These markers will be useful for the assessment of genetic variation for this species.

Original languageEnglish
Pages (from-to)893-895
Number of pages3
JournalMolecular Ecology Notes
Volume5
Issue number4
DOIs
Publication statusPublished - 2005 Dec 1

Keywords

  • Endangered fish
  • Hucho perryi
  • Japanese huchen
  • Microsatellite DNA

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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