Isolation and characterization of low-indole-3-acetic acid-producing mutants from bradyrhizobium elkanii

Ken Yagi, Taku Matsumoto, Tetsuya Chujo, Hideaki Nojiri, Toshio Omori, Kiwamu Minamisawa, Makoto Nishiyama, Hisakazu Yamane

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.

Original languageEnglish
Pages (from-to)1359-1364
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number7
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Biosynthesis of indole-3-acetic acid
  • Bradyrhizobium elkanii
  • Indole-3-pyruvic acid decarboxylase
  • Tn5 mutagenesis

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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