TY - JOUR
T1 - Isolation and characterization of lamprey rhodopsin cDNA
AU - Hisatomi, Osamu
AU - Iwasa, Tatsuo
AU - Tokunaga, Fumio
AU - Yasui, Akira
PY - 1991/2/14
Y1 - 1991/2/14
N2 - Genomic DNA fragments coding a visual pigment of the lamprey were amplified by polymerase chain reaction, using oligonucleotide mixtures as primers. The complete coding region of the cDNA was obtained by separate amplification of both cDNA ends. The deduced amino acid sequence of the coding region showed 78 - 82 % identity with those of rhodopsins of higher vertebrates, but only 43 - 47 % identity with those of human color pigments. The cloned DNA appears to be the cDNA of a lamprey rhodopsin, which is expressed in the "short" photoreceptor cell.
AB - Genomic DNA fragments coding a visual pigment of the lamprey were amplified by polymerase chain reaction, using oligonucleotide mixtures as primers. The complete coding region of the cDNA was obtained by separate amplification of both cDNA ends. The deduced amino acid sequence of the coding region showed 78 - 82 % identity with those of rhodopsins of higher vertebrates, but only 43 - 47 % identity with those of human color pigments. The cloned DNA appears to be the cDNA of a lamprey rhodopsin, which is expressed in the "short" photoreceptor cell.
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U2 - 10.1016/0006-291X(91)91537-M
DO - 10.1016/0006-291X(91)91537-M
M3 - Article
C2 - 1840482
AN - SCOPUS:0025971546
VL - 174
SP - 1125
EP - 1132
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -