Isolation and characterization of lamprey rhodopsin cDNA

Osamu Hisatomi, Tatsuo Iwasa, Fumio Tokunaga, Akira Yasui

    Research output: Contribution to journalArticle

    64 Citations (Scopus)

    Abstract

    Genomic DNA fragments coding a visual pigment of the lamprey were amplified by polymerase chain reaction, using oligonucleotide mixtures as primers. The complete coding region of the cDNA was obtained by separate amplification of both cDNA ends. The deduced amino acid sequence of the coding region showed 78 - 82 % identity with those of rhodopsins of higher vertebrates, but only 43 - 47 % identity with those of human color pigments. The cloned DNA appears to be the cDNA of a lamprey rhodopsin, which is expressed in the "short" photoreceptor cell.

    Original languageEnglish
    Pages (from-to)1125-1132
    Number of pages8
    JournalBiochemical and biophysical research communications
    Volume174
    Issue number3
    DOIs
    Publication statusPublished - 1991 Feb 14

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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