Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae

Takako Itoh, Hiroshige Tsuzuki, Takaaki Katoh, Hiroshi Teraoka, Kohichi Matsumoto, Nobuo Yoshida, Kimio Terazono, Takuo Watanabe, Hideto Yonekura, Hiroshi Yamamoto, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of α-helix and β-sheet contents.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalFEBS Letters
Volume272
Issue number1-2
DOIs
Publication statusPublished - 1990 Oct 15

Keywords

  • Human pancreas
  • Pancreatic stone protein
  • Secondary structure
  • Yeast
  • reg protein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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