Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae

Takako Itoh; Hiroshige Tsuzuki; Takaaki Katoh; Hiroshi Teraoka; Kohichi Matsumoto; Nobuo Yoshida; Kimio Terazono; Takuo Watanabe; Hideto Yonekura; Hiroshi Yamamoto; Hiroshi Okamoto

doi:10.1016/0014-5793(90)80454-Q

Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae

Takako Itoh, Hiroshige Tsuzuki, Takaaki Katoh, Hiroshi Teraoka, Kohichi Matsumoto, Nobuo Yoshida, Kimio Terazono, Takuo Watanabe, Hideto Yonekura, Hiroshi Yamamoto, Hiroshi Okamoto

Surgery

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Abstract

reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of α-helix and β-sheet contents.

Original language	English
Pages (from-to)	85-88
Number of pages	4
Journal	FEBS Letters
Volume	272
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)80454-Q
Publication status	Published - 1990 Oct 15

Keywords

Human pancreas
Pancreatic stone protein
Secondary structure
Yeast
reg protein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(90)80454-Q

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abstract = "reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of α-helix and β-sheet contents.",

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T1 - Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae

AU - Itoh, Takako

AU - Tsuzuki, Hiroshige

AU - Katoh, Takaaki

AU - Teraoka, Hiroshi

AU - Matsumoto, Kohichi

AU - Yoshida, Nobuo

AU - Terazono, Kimio

AU - Watanabe, Takuo

AU - Yonekura, Hideto

AU - Yamamoto, Hiroshi

AU - Okamoto, Hiroshi

PY - 1990/10/15

Y1 - 1990/10/15

N2 - reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of α-helix and β-sheet contents.

AB - reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of α-helix and β-sheet contents.

KW - Human pancreas

KW - Pancreatic stone protein

KW - Secondary structure

KW - Yeast

KW - reg protein

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Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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