Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties

Adel Elbeltagy, Kiyo Nishioka, Hisa Suzuki, Tadashi Sato, Yo Ichiro Sato, Hisao Morisaki, Hisayuki Mitsui, Kiwamu Minamisawa

Research output: Contribution to journalArticlepeer-review

108 Citations (Scopus)

Abstract

Endophytic bacteria were isolated from surface-sterilized stems, seeds, and leaf sheaths of wild and traditionally cultivated rice varieties. Phylogenetic analyses based on 16S rDNA revealed a wide divergence among the isolates. However, the most frequently isolated groups were Methylobacterium sp. in the α-subdivision of Proteobacteria, and Curtobacterium sp. in the high G+ C Gram-positive group. Various phenotypic traits that are expected to be involved in the persistence and functions of the bacteria were analyzed: Most of the isolates from rice excreted pectinase, were motile, and showed an osmotic resistance to 0.6 M sucrose. These traits may be involved in endophytic characteristics in rice. About 50% of the isolates showed a cellulase activity. A few isolates fixed nitrogen, produced indole-3-acetic acid, and formed capsules. These activities were partially correlated with the phylogenetic group.

Original languageEnglish
Pages (from-to)617-629
Number of pages13
JournalSoil Science and Plant Nutrition
Volume46
Issue number3
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Curtobacterium
  • Endophytic bacteria
  • Methylobacterium
  • Pectinase
  • Rice

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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