Isolation and characterization of acetoacetyl-CoA thiolase gene essential for n-decane assimilation in yeast Yarrowia lipolytica

Setsu Yamagami, Toshiya Iida, Yuji Nagata, Akinori Ohta, Masamichi Takagi

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Yarrowia lipolytica is a yeast which can utilize n-alkane as a sole carbon source. We isolated a Y. lipolytica peroxisomal acetoacetyl-CoA thiolase gene, PAT1, by complementation of a mutant that cannot utilize n-decane as a sole carbon source. We found that the putative PAT1 product had conserved features of peroxisomal acetoacetyl-CoA thiolase. We showed that the PAT1 disruptant was not able to grow on n-decane, and that n-decane-inducible acetoacetyl-CoA thiolase activity largely depended on PAT1. The original mutant carried a mutation involving the replacement of Gly382 with Glu. This mutation inactivated the ability of PAT1 to complement the defective n-decane utilization of the disruptant. These results indicate that PAT1 encodes peroxisomal acetoacetyl-CoA thiolase and is essential for n-decane utilization in Y. lipolytica.

Original languageEnglish
Pages (from-to)832-838
Number of pages7
JournalBiochemical and biophysical research communications
Volume282
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Acetoacetyl-CoA thiolase
  • Yarrowia lipolytica
  • n-alkane metabolism

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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