TY - JOUR
T1 - Isolation and Characterization of a Protease Inhibitor from the Marine Gastropod Tugali gigas
AU - Yoshino, Yoshikazu
AU - Muramoto, Koji
AU - Goto, Rina
AU - Kamiya, Hisao
PY - 1993/1/1
Y1 - 1993/1/1
N2 - A protease inhibitor, named tugalistatin, was isolated from the muscle of the marine gastropod Tugali gigas. Tugalistatin inhibited the proteolytic activity of trypsin and α-chymotrypsin. It also suppressed the activity of kallikrein as well as elastase to some extent. On the other hand, it was inactive against metallo-proteases and cysteine proteases. These suggest that tugalistatin is a serine protease inhibitor. In SDS-polyacryamide gel electrophoresis it showed a single band corresponding to 22,000 under reduced conditions. The molecular weight of intact inhibitor was calculated to be 17,000 when checked by FPLC on a Superose 12 column. Amino acid analysis revealed tugalistatin contained a large amount of glutamic acid (14.0%), half-cystine (11.1%), and aspartic acid (9.7%) in the molecule.
AB - A protease inhibitor, named tugalistatin, was isolated from the muscle of the marine gastropod Tugali gigas. Tugalistatin inhibited the proteolytic activity of trypsin and α-chymotrypsin. It also suppressed the activity of kallikrein as well as elastase to some extent. On the other hand, it was inactive against metallo-proteases and cysteine proteases. These suggest that tugalistatin is a serine protease inhibitor. In SDS-polyacryamide gel electrophoresis it showed a single band corresponding to 22,000 under reduced conditions. The molecular weight of intact inhibitor was calculated to be 17,000 when checked by FPLC on a Superose 12 column. Amino acid analysis revealed tugalistatin contained a large amount of glutamic acid (14.0%), half-cystine (11.1%), and aspartic acid (9.7%) in the molecule.
UR - http://www.scopus.com/inward/record.url?scp=85008112598&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008112598&partnerID=8YFLogxK
U2 - 10.2331/suisan.59.1923
DO - 10.2331/suisan.59.1923
M3 - Article
AN - SCOPUS:85008112598
VL - 59
SP - 1923
EP - 1928
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 11
ER -