Isolation and characterization of a novel jumbo phage from leaf litter compost and its suppressive effect on rice seedling rot diseases

Ryota Sasaki, Shuhei Miyashita, Sugihiro Ando, Kumiko Ito, Toshiyuki Fukuhara, Hideki Takahashi

Research output: Contribution to journalArticlepeer-review

Abstract

Jumbo phages have DNA genomes larger than 200 kbp in large virions composed of an icosahedral head, tail, and other adsorption structures, and they are known to be abundant biological substances in nature. In this study, phages in leaf litter compost were screened for their potential to suppress rice seedling rot disease caused by the bacterium Burkholderia glumae, and a novel phage was identified in a filtrate-enriched suspension of leaf litter compost. The phage particles consisted of a rigid tailed icosahedral head and contained a DNA genome of 227,105 bp. The phage could lyse five strains of B. glumae and six strains of Burkholderia plantarii. The phage was named jumbo Burkholderia phage FLC6. Proteomic tree analysis revealed that phage FLC6 belongs to the same clade as two jumbo Ralstonia phages, namely RSF1 and RSL2, which are members of the genus Chiangmaivirus (family: Myoviridae; order: Caudovirales). Interestingly, FLC6 could also lyse two strains of Ralstonia pseudosolanacearum, the causal agent of bacterial wilt, suggesting that FLC6 has a broad host range that may make it especially advantageous as a bio-control agent for several bacterial diseases in economically important crops. The novel jumbo phage FLC6 may enable leaf litter compost to suppress several bacterial diseases and may itself be useful for controlling plant diseases in crop cultivation.

Original languageEnglish
Article number591
JournalViruses
Volume13
Issue number4
DOIs
Publication statusPublished - 2021 Apr

Keywords

  • Biocontrol agent
  • Broad host range
  • Burkholderia
  • Jumbo phage
  • Leaf litter compost
  • Phage therapy
  • Ralstonia

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Fingerprint

Dive into the research topics of 'Isolation and characterization of a novel jumbo phage from leaf litter compost and its suppressive effect on rice seedling rot diseases'. Together they form a unique fingerprint.

Cite this