Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II

Takashi Umehara; Satoshi Kida; Tohru Yamamoto; Masami Horikoshi

doi:10.1016/0378-1119(95)00634-6

Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II

Takashi Umehara, Satoshi Kida, Tohru Yamamoto, Masami Horikoshi

University Hospital

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

We report the isolation of a cDNA encoding a new type of transcription factor S-II, termed h-SII-T1, from a human library. The mRNA corresponding to the clone is highly expressed in testis and ovary. Comparison of the deduced amino acid (aa) sequence with those of other S-II molecules shows that (i) the C-terminal zinc finger (Zf) domain is highly conserved, and (ii) the central segment is most similar to that of the rat testis-specific S-II. Further analyses of the hS-II-T1 aa sequence indicate that its N-terminal sequence exhibits similarity to eubacterial σ⁵⁴. The significance of tissue-specific S-II molecules for the regulation of transcription elongation is discussed.

Original language	English
Pages (from-to)	297-302
Number of pages	6
Journal	Gene
Volume	167
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(95)00634-6
Publication status	Published - 1995 Dec 29

Keywords

Antitermination
RNA polymerase II
RPB9
pausing
sigma similarity

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(95)00634-6

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title = "Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II",

abstract = "We report the isolation of a cDNA encoding a new type of transcription factor S-II, termed h-SII-T1, from a human library. The mRNA corresponding to the clone is highly expressed in testis and ovary. Comparison of the deduced amino acid (aa) sequence with those of other S-II molecules shows that (i) the C-terminal zinc finger (Zf) domain is highly conserved, and (ii) the central segment is most similar to that of the rat testis-specific S-II. Further analyses of the hS-II-T1 aa sequence indicate that its N-terminal sequence exhibits similarity to eubacterial σ54. The significance of tissue-specific S-II molecules for the regulation of transcription elongation is discussed.",

keywords = "Antitermination, RNA polymerase II, RPB9, pausing, sigma similarity",

author = "Takashi Umehara and Satoshi Kida and Tohru Yamamoto and Masami Horikoshi",

note = "Funding Information: We thank Dr. S. Elledge for a human cDNA library and the materials for two-hybrid screening, S. Kitao for initial screening and Dr. P.A. Weil, T. Suzuki, K. Hasegawa and S. Ijuin for critical reading of the manuscript. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, and by grants from Research",

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doi = "10.1016/0378-1119(95)00634-6",

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T1 - Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II

AU - Umehara, Takashi

AU - Kida, Satoshi

AU - Yamamoto, Tohru

AU - Horikoshi, Masami

N1 - Funding Information: We thank Dr. S. Elledge for a human cDNA library and the materials for two-hybrid screening, S. Kitao for initial screening and Dr. P.A. Weil, T. Suzuki, K. Hasegawa and S. Ijuin for critical reading of the manuscript. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, and by grants from Research

PY - 1995/12/29

Y1 - 1995/12/29

N2 - We report the isolation of a cDNA encoding a new type of transcription factor S-II, termed h-SII-T1, from a human library. The mRNA corresponding to the clone is highly expressed in testis and ovary. Comparison of the deduced amino acid (aa) sequence with those of other S-II molecules shows that (i) the C-terminal zinc finger (Zf) domain is highly conserved, and (ii) the central segment is most similar to that of the rat testis-specific S-II. Further analyses of the hS-II-T1 aa sequence indicate that its N-terminal sequence exhibits similarity to eubacterial σ54. The significance of tissue-specific S-II molecules for the regulation of transcription elongation is discussed.

AB - We report the isolation of a cDNA encoding a new type of transcription factor S-II, termed h-SII-T1, from a human library. The mRNA corresponding to the clone is highly expressed in testis and ovary. Comparison of the deduced amino acid (aa) sequence with those of other S-II molecules shows that (i) the C-terminal zinc finger (Zf) domain is highly conserved, and (ii) the central segment is most similar to that of the rat testis-specific S-II. Further analyses of the hS-II-T1 aa sequence indicate that its N-terminal sequence exhibits similarity to eubacterial σ54. The significance of tissue-specific S-II molecules for the regulation of transcription elongation is discussed.

KW - Antitermination

KW - RNA polymerase II

KW - RPB9

KW - pausing

KW - sigma similarity

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DO - 10.1016/0378-1119(95)00634-6

M3 - Article

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AN - SCOPUS:0029595315

VL - 167

SP - 297

EP - 302

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -

Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II

Abstract

Keywords

ASJC Scopus subject areas

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