Isolation and 18S ribosomal DNA gene sequences of Marteilioides chungmuensis (Paramyxea), an ovarian parasite of the Pacific oyster Crassostrea gigas

Naoki Itoh, Tadashi Oda, Tomoyoshi Yoshinaga, Kazuo Ogawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To develop sensitive detection techniques with the aim of elucidating the life cycle of Marteilioides chungmuensis, an intracellular paramyxean infecting the ovary of the Pacific oyster Crassostrea gigas, we isolated the parasite at the sporont stage from infected oysters using a freeze-thaw procedure at -20°C and differential centrifugations in discontinuous sucrose and Percoll gradients. DNA was extracted from the isolated sporonts, and a PCR amplicon of 18S small subunit ribosomal RNA gene DNA was partially sequenced. In situ hybridization using 3 parasite-specific probes designed from the obtained sequence successfully detected parasite cells in infected oysters, and cofirmed that the sequenced DNA was derived from M. chungmuensis.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalDiseases of Aquatic Organisms
Volume54
Issue number2
DOIs
Publication statusPublished - 2003 Mar 31

Keywords

  • 18S ribosomal DNA gene sequence
  • Crassostrea gigas
  • In situ hybridization
  • Isolation
  • Marteilioides chungmuensis
  • Pacific oyster
  • Paramyxea

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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