Isoform-specific retinoid-X receptor (RXR) antibodies detect differential expression of RXR proteins in the pituitary gland

Akira Sugawara, Paul M. Yen, Yanping Qi, Ronald M. Lechan, William W. Chin

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

There are three known isoforms of the retinoid-X receptor (RXR): RXR α, RXR β, and RXR γ. RXR α and RXR β messenger RNAs are widely expressed, whereas RXR γ messenger RNA is restricted to only a few tissues, including embryonic pituitary gland. Little is known about the level of expression and cell distribution of RXR proteins in the adult pituitary gland. To examine these issues further, we raised isoform-specific polyclonal antibodies against each of the known mouse RXR isoforms using synthetic peptides containing isoform-specific epitopes from the amino-terminal region. The specificity of each antibody was confirmed by immunoprecipitation, Western immunoblot analysis, and electrophoretic mobility shift assay with supershift studies of in vitro translated RXR isoforms. Immunocytochemical analysis showed that anti-RXR α and anti-RXR β antisera stained the nuclei of most pituitary cells. In contrast, anti-RXR γ antiserum stained the nuclei of only a few cells throughout the pituitary. In the hypothyroid state, however, a marked increase in both the number and density of RXR γ-immunostained nuclei were observed compared to those in the euthyroid state. Double immunostaining studies of hypothyroid rat pituitary with antibodies against pituitary hormones indicated that RXR γ protein was predominantly expressed in thyrotropes. Antibody supershift experiments using nuclear extracts of adult rat whole pituitary and rodent pituitary cell lines showed that anti-RXR γ antibody could alter the mobility of protein-DNA complexes formed only from nuclear extracts of rat whole pituitary and thyrotropic TtT-97 cells. In contrast, anti-RXR α and anti-RXR β antibodies could supershift protein-DNA complexes formed from nuclear extracts of all cell lines tested. RXR γ protein expression in TtT-97 cells also was observed by Western immunoblot analyses. Therefore, there is thyrotrope-predominant expression of RXR γ protein. We speculate that RXR γ may play a role in the regulation of thyroid hormone target genes in thyrotropes and possibly cell type differentiation in the pituitary.

Original languageEnglish
Pages (from-to)1766-1774
Number of pages9
JournalEndocrinology
Volume136
Issue number4
DOIs
Publication statusPublished - 1995 Apr

ASJC Scopus subject areas

  • Endocrinology

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