TY - JOUR
T1 - Isoform specific phosphorylation of protein phosphatase 2C expressed in COS7 cells
AU - Kobayashi, Takayasu
AU - Kusuda, Kazuyuki
AU - Ohnishi, Motoko
AU - Wang, Hong
AU - Ikeda, Shoko
AU - Hanada, Masahito
AU - Yanagawa, Yuchio
AU - Tamura, Shinri
N1 - Funding Information:
We thank Mr. Kimio Konno for technical assistance and Ms. Noriko Yamagata for secretarial assistance. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, ONO Medical Research Foundation, Smoking Research Foundation, Nakayama Foundation of Human Science and the 1st Toyota High-tec Research Grant Program.
PY - 1998/7/3
Y1 - 1998/7/3
N2 - Of the six distinct isoforms of mouse protein phosphatase 2C (PP2C) (α, β-1, β-2, β-3, β-4 and β-5), PP2Cα was specifically phosphorylated on the serine residue(s) when expressed in COS7 cells. Analysis of phosphorylation sites using site-directed mutagenesis demonstrated that Ser- 375 and/or Ser377 were phosphorylated in vivo. These serine residues were the sites of phosphorylation by casein kinase II in vitro. Phosphorylation of PP2Cα was enhanced two-fold by the addition of okadaic acid to the culture medium, but addition of cyclosporin A had no such effect. These results suggest that the expressed PP2Cα is phosphorylated by a casein kinase II- like protein kinase and dephosphorylated by PP1 and/or PP2A in COS7 cells.
AB - Of the six distinct isoforms of mouse protein phosphatase 2C (PP2C) (α, β-1, β-2, β-3, β-4 and β-5), PP2Cα was specifically phosphorylated on the serine residue(s) when expressed in COS7 cells. Analysis of phosphorylation sites using site-directed mutagenesis demonstrated that Ser- 375 and/or Ser377 were phosphorylated in vivo. These serine residues were the sites of phosphorylation by casein kinase II in vitro. Phosphorylation of PP2Cα was enhanced two-fold by the addition of okadaic acid to the culture medium, but addition of cyclosporin A had no such effect. These results suggest that the expressed PP2Cα is phosphorylated by a casein kinase II- like protein kinase and dephosphorylated by PP1 and/or PP2A in COS7 cells.
KW - Okadaic acid
KW - Phosphorylation
KW - Protein phosphatase 2C
KW - Site-directed mutagenesis
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U2 - 10.1016/S0014-5793(98)00604-8
DO - 10.1016/S0014-5793(98)00604-8
M3 - Article
C2 - 9688543
AN - SCOPUS:0032479194
VL - 430
SP - 222
EP - 226
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -