Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10-5-10-10 M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10-11 M), secretin (0.5x 10-6 M) or carbachol (10-6 M), or by a maximal dose of vasoactive intestinal peptide (10-8 M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.
- exocrine pancreas
- rat pancreatic acini
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)