Islet Amyloid Polypeptide has No Effect on Amylase Release from Rat Pancreatic Acini Stimulated by CCK-8, Secretin, Carbachol and VIP

Yoshifumi Kikuchi; Masaru Koizumi; Tooru Shimosegawa; Jyunya Kashimura; Susumu Suzuki; Takayoshi Toyota

doi:10.1620/tjem.165.41

Islet Amyloid Polypeptide has No Effect on Amylase Release from Rat Pancreatic Acini Stimulated by CCK-8, Secretin, Carbachol and VIP

Yoshifumi Kikuchi, Masaru Koizumi, Tooru Shimosegawa, Jyunya Kashimura, Susumu Suzuki, Takayoshi Toyota

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10^-5-10^-10 M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10^-11 M), secretin (0.5x 10^-6 M) or carbachol (10^-6 M), or by a maximal dose of vasoactive intestinal peptide (10^-8 M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.

Original language	English
Pages (from-to)	41-48
Number of pages	8
Journal	Tohoku Journal of Experimental Medicine
Volume	165
Issue number	1
DOIs	https://doi.org/10.1620/tjem.165.41
Publication status	Published - 1991

Keywords

IAPP
exocrine pancreas
rat pancreatic acini

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1620/tjem.165.41

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Islet Amyloid Polypeptide has No Effect on Amylase Release from Rat Pancreatic Acini Stimulated by CCK-8, Secretin, Carbachol and VIP. / Kikuchi, Yoshifumi; Koizumi, Masaru; Shimosegawa, Tooru; Kashimura, Jyunya; Suzuki, Susumu; Toyota, Takayoshi.

In: Tohoku Journal of Experimental Medicine, Vol. 165, No. 1, 1991, p. 41-48.

Research output: Contribution to journal › Article › peer-review

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abstract = "Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10-5-10-10 M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10-11 M), secretin (0.5x 10-6 M) or carbachol (10-6 M), or by a maximal dose of vasoactive intestinal peptide (10-8 M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.",

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AU - Koizumi, Masaru

AU - Shimosegawa, Tooru

AU - Kashimura, Jyunya

AU - Suzuki, Susumu

AU - Toyota, Takayoshi

PY - 1991

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N2 - Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10-5-10-10 M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10-11 M), secretin (0.5x 10-6 M) or carbachol (10-6 M), or by a maximal dose of vasoactive intestinal peptide (10-8 M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.

AB - Islet amyloid polypeptide (IAPP), a novel peptide recently isolated from the amyloid substance of pancreatic islets, has been localized in normal islet B cell granules, suggesting its physiological action in exocrine pancreatic function. In the present study, the direct effect of IAPP on secretagogue-stimulated pancreatic enzyme secretion was investigated using dispersed rat pancreatic acini. Various concentrations of IAPP (10-5-10-10 M) showed no significant influence on amylase release stimulated either by submaximal doses of CCK-8 (10-11 M), secretin (0.5x 10-6 M) or carbachol (10-6 M), or by a maximal dose of vasoactive intestinal peptide (10-8 M). These results may provide negative evidence for a physiological significance of IAPP in rat pancreatic enzyme secretion.

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Islet Amyloid Polypeptide has No Effect on Amylase Release from Rat Pancreatic Acini Stimulated by CCK-8, Secretin, Carbachol and VIP

Abstract

Keywords

ASJC Scopus subject areas

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