Is GHRH receptor essential to GHRP-2-induced GH secretion in primary cultured rat pituitary cells?

Sang Gun Roh, Chen Chen, Ki Choon Choi, Yogendra Shrestha, Shin Ichi Sasaki

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

It is still controversial in rat whether the stimulation of GH secretion by GH-releasing peptides (GHRP) requires both GHRP receptor (GHRP-R) and GH-releasing hormone receptor (GHRH-R). To clarify this issue, we have postulated that inhibition of GHS-R or GHRH-R gene transcription should block GHRP-2-induced GH secretion. Rat pituitary cells were incubated for 3 days in the presence or absence of antisense 18-mer phosphorothiate oligonucleotides (ONs) complementary to the codon region of GHS-R or GHRH-R mRNAs. A significant decrease in GHRH-R and GHS-R mRNA levels was found in corresponding antisense-treated cells compared with the control cells treated with sense ON. Treatment with antisense GHS-R ON reduced (but not abolished) GHRP-2-induced GH secretion although GHRH-induced GH secretion was not altered. GHRH-stimulated GH secretion was totally abolished by the treatment with antisense GHRH-R ON, whereas GHRP-2 induced GH secretion was not affected. Treatment of cells with both GHS-R and GHRH-R ONs however completely inhibited GHRH and GHRP-2-stimulated GH secretion. These results suggest that GHRH-R is vital for GHRH-induced GH secretion but only partially involved in GHRP-2-stimulated GH secretion under the condition of down-regulation of GHS-R gene transcription.

Original languageEnglish
Pages (from-to)1964-1967
Number of pages4
JournalEndocrinology
Volume143
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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