Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation

Yuji Tanaka, Dong Shi, Akira Kubota, Yoshimasa Takano, Nobuo Fuse, Masayuki Yamato, Teruo Okano, Kohji Nishida

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tissue engineering and transplantation of autogenic grafts have been widely investigated for solving problems on current allograft treatments (i.g., donor shortage and rejection). However, it is difficult to obtain an autogenic corneal stromal replacement that is composed of transparent, tough, and thick collagen constructs by current cell culture-based tissue engineering. Aim of this study is to develop transparent dermis for an autogenic corneal stroma transplantation. This study examined dehydration at 4-8 °C and carbodiimide cross-linking on cloudy rabbit dermis (approx. 1.8%-3.8% light transmittance at 550. nm) for dermis optical clearing. Transparency of dehydrated rabbit dermis was founded to be approx. 37.9%-41.4% at 550. nm. Additional cross-linking treatment on dehydrated dermis prevented from swelling and clouding in saline, and improved its transparency to be 56.9% at 550. nm. Rabbit corneal epithelium was found to regenerate on optically cleared dermis . in vitro. Furthermore, no abnormal biological response (i.e., inflammation, vascularization, and the barrier defect of epithelia) or no optical functional change on optically cleared dermis was observed during its 4-week autogenic transplantation into rabbit corneal stromal pocket.

Original languageEnglish
Pages (from-to)6764-6772
Number of pages9
JournalBiomaterials
Volume32
Issue number28
DOIs
Publication statusPublished - 2011 Oct

Keywords

  • Autogenic transplantation
  • Chemical cross-linking
  • Corneal stroma
  • Dermis
  • Optical clearing

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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  • Cite this

    Tanaka, Y., Shi, D., Kubota, A., Takano, Y., Fuse, N., Yamato, M., Okano, T., & Nishida, K. (2011). Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation. Biomaterials, 32(28), 6764-6772. https://doi.org/10.1016/j.biomaterials.2011.05.081