Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide

Takashi Matsuyama, Kim Domyoung, Mitsuo Umetsu, Taiji Ikawa, Mamoru Yamanishi, Nobuhiro Ishida, Izumi Kumagai, Haruo Takahashi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, "SSSWWSWWWW" (SW1) or "SWWWWSWWWW" (SW2), containing serine (S) and tryptophan (W) at the C terminus localized at the IL/water interface. While GFPs without W-rich peptide distributed only in water phase, SW1- and SW2-GFPs were accumulated at the interface. The localization of SW1-GFP showed biphasic behavior, and most distinctive localization was observed at 7.1. μM. The localization of SW2-GFP presumably occurred at largely lower concentration (≤ 0.5 μM) than that of SW1-GFP, which difference was due to the higher hydrophobicity of SW2 peptide.

Original languageEnglish
Pages (from-to)160-165
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number2
DOIs
Publication statusPublished - 2012 Feb

Keywords

  • Dynamic equilibrium
  • Interface
  • Ionic liquid
  • Peptide
  • Self organization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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