Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman

Takaya Oikawa, Yasuhiro Ishimaru, Shintaro Munemasa, Yusuke Takeuchi, Kento Washiyama, Shin Hamamoto, Nobuyuki Yoshikawa, Yoshiyuki Mutara, Nobuyuki Uozumi, Minoru Ueda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The circadian leaf opening and closing (nyctinasty) of Fabaceae has attracted scientists’ attention since the era of Charles Darwin. Nyctinastic movement is triggered by the alternate swelling and shrinking of motor cells at the base of the leaf. This, in turn, is facilitated by changing osmotic pressures brought about by ion flow through anion and potassium ion channels. However, key regulatory ion channels and molecular mechanisms remain largely unknown. Here, we identify three key ion channels in mimosoid tree Samanea saman: the slow-type anion channels, SsSLAH1 and SsSLAH3, and the Shaker-type potassium channel, SPORK2. We show that cell-specific circadian expression of SsSLAH1 plays a key role in nyctinastic leaf opening. In addition, SsSLAH1 co-expressed with SsSLAH3 in flexor (abaxial) motor cells promoted leaf opening. We confirm the importance of SLAH1 in leaf movement using SLAH1-impaired Glycine max. Identification of this “master player” advances our molecular understanding of nyctinasty. Observations of the daily opening and closing of leaves (nyctinasty) in the 18th century provided some of the first evidence of a biological clock. Oikawa et al. show that circadian regulation of the anion channel SsSLAH1 in the mimosoid tree is the likely molecular mechanism behind nyctinasty.

Original languageEnglish
Pages (from-to)2230-2238.e7
JournalCurrent Biology
Volume28
Issue number14
DOIs
Publication statusPublished - 2018 Jul 23

Keywords

  • Samanea saman
  • circadian rhythm
  • ion channel
  • nyctinasty

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman'. Together they form a unique fingerprint.

  • Cite this

    Oikawa, T., Ishimaru, Y., Munemasa, S., Takeuchi, Y., Washiyama, K., Hamamoto, S., Yoshikawa, N., Mutara, Y., Uozumi, N., & Ueda, M. (2018). Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman. Current Biology, 28(14), 2230-2238.e7. https://doi.org/10.1016/j.cub.2018.05.042