Calcitonin-secreting cells were isolated from ultimobranchial gland of the chick embryos (18-20 days after fertilization). Using whole-cell patch electrode, the membrane currents of the dissociated cells were recorded. With depolarization, tetrodotoxin-sensitive Na+ current was produced which was followed by slowly developing outward K+ current. These cells were positively stained with anticalcitonin antibody (#7701R1) raised in the rabbit. In some cells, no measurable Na+ currents were evoked and the stainings with the antibody were faint or negative.
|Number of pages||2|
|Issue number||SUPPL. 2|
|Publication status||Published - 1988 Jan 1|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)