Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification

Tomonori Katsuyama, Tomo Sugawara, Masanobu Tatsumi, Yoshiteru Oshima, Walter J. Gehring, Toshiro Aigaki, Shoichiro Kurata

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

How organ identity is determined is a fundamental question in developmental biology. In Drosophila, field-specific selector genes, such as eyeless (ey) for eyes and vestigial (vg) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye (wge), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge-induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-(Antennapedia) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent (eya), a control gene for eye development downstream of ey, in eye discs. In contrast to vg, however, overexpression of wge inhibited EY-mediated expression of eya. Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc. These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes.

Original languageEnglish
Pages (from-to)15918-15923
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number44
DOIs
Publication statusPublished - 2005 Nov 1

Keywords

  • Determination
  • Epigenetic regulation
  • Imaginal disc
  • Organ identity

ASJC Scopus subject areas

  • General

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