Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection

Toshio Hattori; Atsushi Koito; Kiyoshi Takatsuki; Hiroshi Kido; Nobuhiko Katunuma

doi:10.1016/0014-5793(89)80429-6

Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection

Toshio Hattori, Atsushi Koito, Kiyoshi Takatsuki, Hiroshi Kido, Nobuhiko Katunuma

International Research Institute of Disaster Science (IRIDeS)

Research output: Contribution to journal › Article › peer-review

128 Citations (Scopus)

Abstract

Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.

Original language	English
Pages (from-to)	48-52
Number of pages	5
Journal	FEBS Letters
Volume	248
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(89)80429-6
Publication status	Published - 1989 May 8

Keywords

HIV-1
Neutralization
Trypstatin
Tryptase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection",

abstract = "Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.",

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AU - Katunuma, Nobuhiko

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AB - Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.

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