Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection

Toshio Hattori; Atsushi Koito; Kiyoshi Takatsuki; Hiroshi Kido; Nobuhiko Katunuma

doi:10.1016/0014-5793(89)80429-6

Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection

Toshio Hattori, Atsushi Koito, Kiyoshi Takatsuki, Hiroshi Kido, Nobuhiko Katunuma

International Research Institute of Disaster Science (IRIDeS)

Research output: Contribution to journal › Article › peer-review

130 Citations (Scopus)

Abstract

Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.

Original language	English
Pages (from-to)	48-52
Number of pages	5
Journal	FEBS Letters
Volume	248
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(89)80429-6
Publication status	Published - 1989 May 8

Keywords

HIV-1
Neutralization
Trypstatin
Tryptase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0014-5793(89)80429-6

Cite this

@article{42cffb77cf884f899b0e416a8fde9d42,

title = "Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection",

abstract = "Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.",

keywords = "HIV-1, Neutralization, Trypstatin, Tryptase",

author = "Toshio Hattori and Atsushi Koito and Kiyoshi Takatsuki and Hiroshi Kido and Nobuhiko Katunuma",

year = "1989",

month = may,

day = "8",

doi = "10.1016/0014-5793(89)80429-6",

language = "English",

volume = "248",

pages = "48--52",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection

AU - Hattori, Toshio

AU - Koito, Atsushi

AU - Takatsuki, Kiyoshi

AU - Kido, Hiroshi

AU - Katunuma, Nobuhiko

PY - 1989/5/8

Y1 - 1989/5/8

N2 - Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.

AB - Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.

KW - HIV-1

KW - Neutralization

KW - Trypstatin

KW - Tryptase

UR - http://www.scopus.com/inward/record.url?scp=0024554268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024554268&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(89)80429-6

DO - 10.1016/0014-5793(89)80429-6

M3 - Article

C2 - 2470618

AN - SCOPUS:0024554268

VL - 248

SP - 48

EP - 52

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -

Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this