Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1

Akira Asai, Fumitaka Okajima, Yasushi Nakajima, Mototsugu Nagao, Kiyotaka Nakagawa, Teruo Miyazawa, Shinichi Oikawa

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Increasing evidence indicates that phospholipid oxidation plays important roles in atherosclerosis. Here, we investigated the involvement of Rho-family GTPases inphosphatidylcholine hydroperoxide (PCOOH)-induced THP-1 cell adhesion to ICAM-1. Isoprenoid depletion by fluvastatin and geranylgeranyltransferase inhibition by GGTI-286 suppressed PCOOH-induced cell adhesion to ICAM-1 and F-actin-rich membrane protrusion formation. Pull-down assays demonstrated the activation of Rac1 and Rac2 in PCOOH-treated cells. Pan-Rho-family GTPase inhibitor Clostridium difficile toxin B, Rac-specific inhibitor NSC23776, and RNA interference of the Rac isoforms suppressed the cell adhesion. These findings indicate the involvement of Rac GTPase activation in PCOOH-induced cell adhesion to ICAM-1 via actin reorganization.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalBiochemical and biophysical research communications
Volume406
Issue number2
DOIs
Publication statusPublished - 2011 Mar 11

Keywords

  • Actin
  • Cell adhesion
  • Monocyte
  • Phosphatidylcholine hydroperoxide
  • Phospholipid oxidation
  • Rac

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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