1. A high performance liquid chromatography (HPLC) using 9-anthryldiazomethane (ADAM) as a fluorescent reagent was employed to detemine the levels of endogenous prostaglandins (PGs) in the central nervous system, gonad, gill and hemolymph of the scallop, and the authors have also verified the involvement of PGs during spawning induced by u.v. ray-irradiated seawater. 2. PGF2α, PGE2, PGD2, 6-keto-PGF1α. and TXB2 were identified in all tissue and hemolymph, while no PGD2 was found in the hemolymph by HPLC. 3. PGF2α, PGE2 and PGD2 levels in the ovary were about four times as much as those in the testis during the spawning season. 4. PGF2α, PGE2 and PGD2 levels in the ovary decreased during spawning, while, on the contrary, those in the testis increased during spawning. No changes of PGs levels were observed in the central nervous system. 5. These results suggest the possibility that PGF2α and PGE2 are, especially, implicated in the spawning of the scallop; however, they also indicate that a difference between the functional mechanism of PGs in the ovary and that in the testis exists during spawning.
|Number of pages||7|
|Journal||Comparative Biochemistry and Physiology. Part C, Comparative|
|Publication status||Published - 1989|
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