Abstract
Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27 h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1 mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.
| Original language | English |
|---|---|
| Pages (from-to) | 238-241 |
| Number of pages | 4 |
| Journal | Journal of Plant Physiology |
| Volume | 167 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Feb 15 |
Keywords
- Lotus japonicus
- Nitrate inhibition
- Nitrate reductase
- Nitric oxide
- Nitrogenase activity
ASJC Scopus subject areas
- Physiology
- Agronomy and Crop Science
- Plant Science