Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules

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48 Citations (Scopus)

Abstract

Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27 h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1 mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.

Original languageEnglish
Pages (from-to)238-241
Number of pages4
JournalJournal of Plant Physiology
Volume167
Issue number3
DOIs
Publication statusPublished - 2010 Feb 15

Keywords

  • Lotus japonicus
  • Nitrate inhibition
  • Nitrate reductase
  • Nitric oxide
  • Nitrogenase activity

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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