Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules

Kazuhisa Kato; Koki Kanahama; Yoshinori Kanayama

doi:10.1016/j.jplph.2009.08.006

Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules

Kazuhisa Kato, Koki Kanahama, Yoshinori Kanayama

AGR - Biological Resource Sciences

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27 h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1 mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.

Original language	English
Pages (from-to)	238-241
Number of pages	4
Journal	Journal of Plant Physiology
Volume	167
Issue number	3
DOIs	https://doi.org/10.1016/j.jplph.2009.08.006
Publication status	Published - 2010 Feb 15

Keywords

Lotus japonicus
Nitrate inhibition
Nitrate reductase
Nitric oxide
Nitrogenase activity

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

Access to Document

10.1016/j.jplph.2009.08.006

Fingerprint Dive into the research topics of 'Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules'. Together they form a unique fingerprint.

Cite this

@article{e137d09cf76d4a3e82c986b98731ba41,

title = "Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules",

abstract = "Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27 h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1 mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.",

keywords = "Lotus japonicus, Nitrate inhibition, Nitrate reductase, Nitric oxide, Nitrogenase activity",

author = "Kazuhisa Kato and Koki Kanahama and Yoshinori Kanayama",

year = "2010",

month = feb,

day = "15",

doi = "10.1016/j.jplph.2009.08.006",

language = "English",

volume = "167",

pages = "238--241",

journal = "Journal of Plant Physiology",

issn = "0176-1617",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "3",

}

TY - JOUR

T1 - Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules

AU - Kato, Kazuhisa

AU - Kanahama, Koki

AU - Kanayama, Yoshinori

PY - 2010/2/15

Y1 - 2010/2/15

N2 - Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27 h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1 mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.

AB - Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27 h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1 mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.

KW - Lotus japonicus

KW - Nitrate inhibition

KW - Nitrate reductase

KW - Nitric oxide

KW - Nitrogenase activity

UR - http://www.scopus.com/inward/record.url?scp=72049132368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72049132368&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2009.08.006

DO - 10.1016/j.jplph.2009.08.006

M3 - Article

C2 - 19733934

AN - SCOPUS:72049132368

VL - 167

SP - 238

EP - 241

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 3

ER -

Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this