Involvement of Na+/H+ exchangers in induction of cyclooxygenase-2 by vacuolar-type (H+)-ATPase inhibitors in RAW 264 cells

Fumitaka Kamachi, Maiko Yanai, Hyun Seung Ban, Kenji Ishihara, Jang Ja Hong, Kazuo Ohuchi, Noriyasu Hirasawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the mouse macrophage-like cell line RAW 264, vacuolar-type (H+)-ATPase (V-ATPase) inhibitors, bafilomycin A1 and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) and nuclear factor-κB, and by inhibitors of Na+/H+ exchangers (NHEs). The bafilomycin A1-induced activation of JNK but not degradation of IκB-α was suppressed by NHE inhibitors and by an inhibitor of Na+/Ca2+ exchanger SN-6. These results suggested that V-ATPase inhibitors induce the expression of COX-2 via NHE-dependent and -independent pathways.

Original languageEnglish
Pages (from-to)4633-4638
Number of pages6
JournalFEBS Letters
Volume581
Issue number24
DOIs
Publication statusPublished - 2007 Oct 2

Keywords

  • Cyclooxygenase-2
  • Na/H exchanger
  • RAW 264 cell line
  • Vacuolar-type (H)-ATPase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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