Involvement of lipoxygenase pathway in docosapentaenoic acid-induced inhibition of platelet aggregation

S. Akiba, T. Murata, K. Kitatani, T. Sato

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

The effects of doeosapentaenoic acid (DPA) on platelet aggregation and arachidonic acid metabolism were studied in comparison to those of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Collagen- or arachidonic acid-stimulated platelet aggregation was inhibited dose-dependently by n-3 fatty acids, among which DPA was the most potent inhibitor. These fatty acids inhibited U46619-induced aggregation but to almost the same extent. No effect of the acids on thrombin-induced aggregation was observed. Furthermore, these fatty acids suppressed thromboxane A2 formation by platelets which were exposed to collagen or thrombin, or by platelets to which arachidonic acid was added. In these experiments also, DPA was the most potent inhibitor, whereas DHA was the most effective inhibitor of cyclooxygenase-1 activity. DPA enhanced formation of 12-hydroxyeicosatetraenoic acid in response to collagen or from arachidonic acid by intact platelets, while the other two acids had less of an effect. These results suggest that DPA possesses potent activity for interfering with the cyclooxygenase pathway and accelerating the lipoxygenase pathway, thus inhibiting platelet aggregation most effectively.

Original languageEnglish
Pages (from-to)1293-1297
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number11
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • 12-hydroxyeicosatetraenoic acid
  • Cyclooxygenase
  • Docosapentaenoic acid
  • Lipoxygenase
  • Platelet aggregation
  • Thromboxane A

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Involvement of lipoxygenase pathway in docosapentaenoic acid-induced inhibition of platelet aggregation'. Together they form a unique fingerprint.

Cite this