Involvement of HMGB1 and HMGB2 proteins in exogenous DNA integration reaction into the genome of HeLa S3 cells

Tetsuya Ueda, Hitoshi Shirakawa, Michiteru Yoshida

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

High mobility group 1 and 2 proteins (HMGB1 and HMGB2) are abundant chromosomal proteins in eukaryotic cells. We examined the involvement of HMGB1 and HMGB2 in nonhomologous illegitimate recombination. The HMGB1 or HMGB2 expression plasmid, carrying the neor gene as a selection marker, was introduced into HeLa S3 cells to obtain stably-transfected cells. The number of G418-resistant colonies was about 10 times the number of colonies of control cells transfected with plasmids not carrying the HMGB genes. The copy number of the stably-integrated neor gene was higher in the cells transfected with the HMGB expression plasmids than in control cells. The exogenous DNA integration was suggested to have occurred by nonhomologous illegitimate recombination. On the contrary, the introduction of the HMGB antisense RNA expression plasmid with a reporter plasmid carrying the neor gene into HeLa S3 cells decreased the number of G418-resistant colonies. These results indicate that HMGB1 and HMGB2 each have a novel function as stimulators of stable integration of plasmid DNA into the host genome and that they may be important for the process of spontaneous DNA integration in living cells.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1593
Issue number1
DOIs
Publication statusPublished - 2002 Dec 16

Keywords

  • HMGB1
  • HMGB2
  • HeLa S3 cell
  • Nonhomologous illegitimate recombination
  • Protein overexpression
  • Stable DNA integration

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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