Involvement of high density lipoprotein as substrate cholesterol for steroidogenesis fasciculo-reticularis cells

Hiroshi Yaguchi, Kazuhiko Tsutsumi, Kazuyuki Shimono, Masao Omura, Hironobu Sasano, Tetsuo Nishikawa

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Adrenocorticosteroids are known to be synthesized from cholesterol which may arise from de novo synthesis or from the uptake of low-density lipoproteins (LDL) or high-density lipoproteins (HDL). LDL is reported to be a main substrate for corticosteroid synthesis by bovine adrenocortical cells, although the role of HDL, which is well known to be used for steroid biosynthesis in rat adrenals, is still obscure. Therefore, we examined the role of HDL in the regulation of corticosteroidogenesis in bovine adrenals in order to clarify whether or not HDL was selectively utilized for corticosteroid synthesis in vitro. The present data demonstrated that HDL and LDL increased cortisol production in a dose-dependent manner in bovine adrenocortical cells in vitro, and also that HDL cholesterol increased cortisol production significantly higher than LDL cholesterol did. Addition of adrenocorticotrophic hormone (ACTH) with HDL to the incubation media enhanced much higher cortisol production than that with LDL in short time incubation. The present data also demonstrated that uptake of 125I-HDL was significantly greater than that of 125I-LDL. Thus, HDL rather than LDL is thought to be the preferred lipoprotein as a source of steroidogenic substrate cholesterol in bovine adrenal fasciculo-reticularis cells.

Original languageEnglish
Pages (from-to)1387-1395
Number of pages9
JournalLife Sciences
Volume62
Issue number16
DOIs
Publication statusPublished - 1998 Mar 13

Keywords

  • Adrenocorticosteroid
  • Bovine
  • High density lipoprotein

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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