Abstract
We previously reported that infection with the periodontopathic bacterium Actinobacillus actinomycetemcomitans induced apoptosis in a mouse macrophage cell line J774.1. In the present study, we examined the involvement of cytochrome c and caspases in the induction of apoptosis in A. actinomycetemcomitans-infected J774.1 cells. Following infection, the expression levels of cytochrome c, and cleaved forms of caspase-3 and caspase-9 in the cells were examined using immunoblot analysis. Cytochrome c was released from mitochondria into the cytoplasm after A. actinomycetemcomitans-infected J774.1 cells were cultured for 6 h, and caspase-3 and caspase-9 were found to be cleaved forms in the cells. Further, caspase-9 activity was markedly increased, and phosphorylated p53 was detected in the cells 30 h following infection. These results suggest that apoptosis in A. actinomycetemcomitans- infected J774.1 cells is regulated by the release of cytochrome c from mitochondria into cytoplasm and the subsequent activation of caspases through phosphorylation of p53.
Original language | English |
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Pages (from-to) | 29-35 |
Number of pages | 7 |
Journal | FEMS Microbiology Letters |
Volume | 233 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2004 Apr 1 |
Externally published | Yes |
Keywords
- Actinobacillus actinomycetemcomitans
- Apoptosis
- Caspases
- Cytochrome c
- Macrophage
- p53
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics