Involvement of cAMP response element-binding protein activation in salivary secretion

Koichi Yamada, Hiroko Inoue, Satoshi Kida, Shoichi Masushige, Tatsuaki Nishiyama, Kenji Mishima, Ichiro Saito

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Objective: Saliva secretion is mediated by cAMP and the calcium signaling pathway in salivary acinar cells. The PKA signaling pathway plays an important role in protein secretion through the activation of cAMP, in fluid secretion through the elevation of intracellular calcium and in the activation of cAMP response element-binding protein (CREB), which is involved in these signaling cascades. In this study, we investigated whether the activation of CREB plays a part in the salivary secretion in mice. Methods: We examined CREB activation by assessing phosphorylation at the serine-133 position using Western blotting. Results: Carbachol (a muscarinic acetylcholine agonist) and isoproterenol (a β-adrenergic agonist) markedly activated CREB in parotid acinar cells. Carbachol and isoproterenol-induced CREB phosphorylation was blocked by atropine (a muscarinic acetylcholine antagonist) and propranolol (a β-adrenergic antagonist), respectively. The PKA inhibitor H89 inhibited CREB activation, but the PLC inhibitor U73122 did not. Moreover, carbachol- and isoproterenol- stimulated amylase secretion from parotid acinar cells was inhibited by H89 and adenoviral dominant-negative CREB. Conclusion: These results indicate that the muscarinic and β-adrenergic activation of CREB was mediated through the PKA pathway and that CREB is involved in protein secretion from parotid acinar cells.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
Issue number1
Publication statusPublished - 2006 Jun
Externally publishedYes


  • CREB
  • Salivary secretion
  • β-Adrenergic receptor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology


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