Involvement of a vascular hypersensitive response in quantitative resistance to Ralstonia solanacearum on tomato rootstock cultivar LS-89

K. Nakaho, S. Seo, K. Ookawa, Y. Inoue, S. Ando, Y. Kanayama, S. Miyashita, H. Takahashi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Ralstonia solanacearum causes bacterial wilt disease in Solanaceae spp. Expression of the Phytophthora inhibitor protease 1 (PIP1) gene, which encodes a papain-like extracellular cysteine protease, is induced in R. solanacearum-inoculated stem tissues of quantitatively resistant tomato cultivar LS-89, but not in susceptible cultivar Ponderosa. Phytophthora inhibitor protease 1 is closely related to Rcr3, which is required for the Cf-2-mediated hypersensitive response (HR) to the leaf mould fungus Cladosporium fulvum and manifestation of HR cell death. However, up-regulation of PIP1 in R. solanacearum-inoculated LS-89 stems was not accompanied by visible HR cell death. Nevertheless, upon electron microscopic examination of inoculated stem tissues of resistant cultivar LS-89, several aggregated materials associated with HR cell death were observed in xylem parenchyma and pith cells surrounding xylem vessels. In addition, the accumulation of electron-dense substances was observed within the xylem vessel lumen of inoculated stems. Moreover, when the leaves of LS-89 or Ponderosa were infiltrated with 106 cells mL−1 R. solanacearum, cell death appeared in LS-89 at 18 and 24 h after infiltration. The proliferation of bacteria in the infiltrated leaf tissues of LS-89 was suppressed to approximately 10–30% of that in Ponderosa, and expression of the defence-related gene PR-2 and HR marker gene hsr203J was induced in the infiltrated tissues. These results indicated that the response of LS-89 is a true HR, and induction of vascular HR in xylem parenchyma and pith cells surrounding xylem vessels seems to be associated with quantitative resistance of LS-89 to R. solanacearum.

Original languageEnglish
Pages (from-to)150-158
Number of pages9
JournalPlant Pathology
Volume66
Issue number1
DOIs
Publication statusPublished - 2017 Jan 1

Keywords

  • HR cell death
  • Phytophthora inhibitor protease 1
  • Ralstonia solanacearum
  • bacterial wilt disease
  • hypersensitive response
  • resistant tomato cultivar LS-89

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

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