Pigment organelles known as melanosomes disperse or aggregate in a melanophore in response to hormones. These movements are mediated by the microtubule motors kinesin 2 and cytoplasmic dynein. However the force generation mechanism of dynein, unlike that of kinesin, is not well understood. In this study, t o address this issue, we in vestigated the dynein mediated aggregation of melanosomes in zebrafish melanophores . We applied the fluctuation theorem of non equilibrium statistical mechanics to estimate forces acting on melanosomes during transport by dynein, given that the energy of a system is related to its fluctuation. Our results demonstrate that multiple force producing units cooperatively transport a single melanosome. Since the force is generated by dynein, this suggests that multiple dyneins carry a single melanosome. C oop erative transport has been reported for other organelles ; multiple motor transport may be a universal mechanism for moving organelle s within the cell.
|Publication status||Published - 2018 Aug 10|
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