TY - JOUR
T1 - Investigation of lipoproteins oxidation mechanisms by the analysis of lipid hydroperoxide isomers
AU - Kato, Shunji
AU - Osuka, Yusuke
AU - Khalifa, Saoussane
AU - Obama, Takashi
AU - Itabe, Hiroyuki
AU - Nakagawa, Kiyotaka
N1 - Funding Information:
This work was supported in part by KAKENHI (grant number 19H02901 to KN) of the Japan Society for the Promotion of Science, Japan. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.Acknowledgments: We thank Junya Ito (Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan) for the technical assistance.
Funding Information:
Funding: This work was supported in part by KAKENHI (grant number 19H02901 to KN) of the Japan Society for the Promotion of Science, Japan. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/10
Y1 - 2021/10
N2 - The continuous formation and accumulation of oxidized lipids (e.g., lipid hydroperox-ides (LOOH)) which are present even in plasma lipoproteins of healthy subjects, are ultimately considered to be linked to various diseases. Because lipid peroxidation mechanisms (i.e., radical, singlet oxygen, and enzymatic oxidation) can be suppressed by certain proper antioxidants (e.g., radical oxidation is efficiently suppressed by tocopherol), in order to suppress lipid peroxidation successfully, the determination of the peroxidation mechanism involved in the formation of LOOH is deemed crucial. In this study, to determine the peroxidation mechanisms of plasma lipoproteins of healthy subjects, we develop novel analytical methods using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine hydroperoxide (PC 16:0/18:2;OOH) and cholesteryl linoleate hydroperoxide (CE 18:2;OOH) isomers. Using the newly developed methods, these PC 16:0/18:2;OOH and CE 18:2;OOH isomers in the low-density lipoprotein (LDL) and high-density lipoprotein (HDL) of healthy subjects are analyzed. Consequently, it is found that predominant PC 16:0/18:2;OOH and CE 18:2;OOH isomers in LDL and HDL are PC 16:0/18:2;9OOH, PC 16:0/18:2;13OOH, CE 18:2;9OOH, and CE 18:2;13OOH, which means that PC and CE in LDL and HDL are mainly oxidized by radical and/or enzymatic oxidation. In conclusion, the insights about the oxidation mechanisms shown in this study would be useful for a more effective suppression of oxidative stress in the human organism.
AB - The continuous formation and accumulation of oxidized lipids (e.g., lipid hydroperox-ides (LOOH)) which are present even in plasma lipoproteins of healthy subjects, are ultimately considered to be linked to various diseases. Because lipid peroxidation mechanisms (i.e., radical, singlet oxygen, and enzymatic oxidation) can be suppressed by certain proper antioxidants (e.g., radical oxidation is efficiently suppressed by tocopherol), in order to suppress lipid peroxidation successfully, the determination of the peroxidation mechanism involved in the formation of LOOH is deemed crucial. In this study, to determine the peroxidation mechanisms of plasma lipoproteins of healthy subjects, we develop novel analytical methods using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine hydroperoxide (PC 16:0/18:2;OOH) and cholesteryl linoleate hydroperoxide (CE 18:2;OOH) isomers. Using the newly developed methods, these PC 16:0/18:2;OOH and CE 18:2;OOH isomers in the low-density lipoprotein (LDL) and high-density lipoprotein (HDL) of healthy subjects are analyzed. Consequently, it is found that predominant PC 16:0/18:2;OOH and CE 18:2;OOH isomers in LDL and HDL are PC 16:0/18:2;9OOH, PC 16:0/18:2;13OOH, CE 18:2;9OOH, and CE 18:2;13OOH, which means that PC and CE in LDL and HDL are mainly oxidized by radical and/or enzymatic oxidation. In conclusion, the insights about the oxidation mechanisms shown in this study would be useful for a more effective suppression of oxidative stress in the human organism.
KW - Lipid hydroperoxide isomers
KW - Lipoproteins
KW - Mass spectrometry
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=85116892568&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85116892568&partnerID=8YFLogxK
U2 - 10.3390/antiox10101598
DO - 10.3390/antiox10101598
M3 - Article
AN - SCOPUS:85116892568
VL - 10
JO - Antioxidants
JF - Antioxidants
SN - 2076-3921
IS - 10
M1 - 1598
ER -
