Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center

Shigeharu Wakana, Tomohiro Suzuki, Tamio Furuse, Kimio Kobayashi, Ikuo Miura, Hideki Kaneda, Ikuko Yamada, Hiromi Motegi, Hideaki Toki, Maki Inoue, Osamu Minowa, Tetsuo Noda, Kazunori Waki, Nobuhiko Tanaka, Hiroshi Masuya, Yuichi Obata

Research output: Contribution to journalReview article

18 Citations (Scopus)

Abstract

A systematic and comprehensive phenotyping platform has been developed by the RIKEN ENU-mutagenesis project between 1999 and 2007. As a result of phenotype screening on this platform, we have discovered about 400 mutants as animal models for human diseases. All information regarding these mouse mutants is now available to the public through our home page (http://www.brc.riken.jp/ lab/gsc/mouse/indexJ.html). In 2008, we reconstructed the existing phenotyping platform and built a new platform. The new system has a hierarchical structure, consisting of a fundamental pipeline that utilizes the existing platform and an additional pipeline, which is optimized for more in-depth phenotyping assays. Using this system, we have started to perform more comprehensive phenotyping of mouse mutants. We have opened this system to Japanese scientists as the Japanese Mouse Clinic. It is anticipated that existing mouse mutants will be reevaluated as disease models by identifying novel phenotypes on the new platform. We will share detailed information about the standard operating procedures (SOPs) of our phenotyping analyses with other related large-scale projects, such as the European Mouse Disease Clinic (EUMODIC) and the German Mouse Clinic (GMC). Moreover, we will contribute to international efforts to standardize mouse phenotype data by sharing annotation of mutant phenotypes, which are made by internationally standardized methods, with other related projects.

Original languageEnglish
Pages (from-to)443-450
Number of pages8
JournalExperimental Animals
Volume58
Issue number5
DOIs
Publication statusPublished - 2009 Dec 1
Externally publishedYes

Keywords

  • Database
  • Mouse
  • Phenotype
  • SOPs
  • Standardization

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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  • Cite this

    Wakana, S., Suzuki, T., Furuse, T., Kobayashi, K., Miura, I., Kaneda, H., Yamada, I., Motegi, H., Toki, H., Inoue, M., Minowa, O., Noda, T., Waki, K., Tanaka, N., Masuya, H., & Obata, Y. (2009). Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center. Experimental Animals, 58(5), 443-450. https://doi.org/10.1538/expanim.58.443